Pokrzywa Lab logo
IIMCB logo

News Archive

November 2023 | New preprint published

C. elegans worms

We have released a preprint elucidating the capacity of floxuridine (FUdR), an anti-cancer pharmaceutical agent, to enhance proteasome resilience in C. elegans under conditions of inhibition or subunit deficits. This phenomenon contributes to the extension of lifespan and facilitates adaptation to cold environments. Furthermore, our investigation reveals the induction of a distinctive detoxification pathway by FUdR.

September 2023 | Our presence at GPI-AP disorders conference

Wojciech at the 1st Polish Conference of Families with Protein Glycosylation Disorders

Our P.I. Wojciech and Ph.D. student Natalia were invited to participate in the 1st Polish Conference of Families with Protein Glycosylation (GPI-AP) Disorders at St. Anne's Mountain. During the event, they presented the possibilities of using the nematode C. elegans as a model organism in human disease studies and discussed with Parents and Patients on the current progress and prospects of rare disease research in Poland.

September 2023 | Our presence
at muscle research workshop

Abhishek at the 24th International C. elegans Conference in Glasgow

Our P.I. Wojciech and two Ph.D. students, Natalia and Anwesha, had the pleasure of attending a workshop on mechanical stress protection at the excellent CECAD institute in Cologne. The meeting allowed us to learn about the latest state-of-the-art research using the C. elegans nematode to better understand muscle functioning, and gave us the opportunity to share our own results and have fruitful scientific discussions.

June 2023 | Our presence
at C. elegans conference

Abhishek at the 24th International C. elegans Conference in Glasgow

Our laboratory, represented by our P.I., Wojciech, postdoc Abhishek and Ph.D. student Natalia, participated in the largest worm conference - the 24th International C. elegans Conference in Glasgow. We presented our research on the cold resistance effect that 5'-fluorodeoxyuridine (FUdR), an anticancer drug, exerts on C. elegans, as well as our results on an ultra-rare mutation in the FEM1C gene that causes a severe neurodevelopmental disorder. We were delighted that both of our posters received a lot of attention, and we look forward to the next meetings with the amazing C. elegans researchers at future conferences.

June 2023 | Our presence
at Rare Disease Congress

Rare Disease Congress

We were delighted to return to Cracow to participate in the 2nd Congress of the Jesteśmy Pod Ścianą Foundation "Rare Diseases - Views from Multiple Perspectives" at Jagiellonian University. Our P.I., Wojciech, and Ph.D. student, Natalia, presented how our research on an uncharacterized neurodevelopmental disorder caused by an ultra-rare mutation in the ubiquitin ligase FEM1C led to the diagnosis and shed some light on our current research focusing on deciphering the mechanism of the disease and developing a potential therapy. We hope that our other projects involving C. elegans and rare mutations will also yield valuable results for patients.

May 2023 | Our presence
at TPD conference in Barcelona

Conference at IRB Barcelona

We are thrilled to share that Natalia, our Ph.D. student, presented our novel web server DEGRONOPEDIA at the "Proximity-inducing pharmacology: Targeted protein degradation and beyond" conference held at the IRB Barcelona - Institute for Research in Biomedicine. The response to the presentation was very positive, and we are excited to continue developing the tool.
You can access DEGRONOPEDIA at degronopedia.com.

April 2023 | New preprint
published

AHCY mutant worm

Since S-adenosylhomocysteine (SAH) extends the lifespan of model organisms, we published a preprint describing our C. elegans model with elevated SAH levels generated by introducing a pathogenic mutation in the enzyme S-adenosylhomocysteine hydrolase (AHCY), which is associated with a rare autosomal recessive disorder of methionine metabolism. Our results demonstrate the potential of AHCY mutant worms as a tool to study AHCY-induced changes in SAH metabolism, as we showed that animals with AHCY mutation exhibited delayed aging accompanied by increased SAH levels.

April 2023 | Ph.D. defense of Katarzyna

Ph.D. defense of Katarzyna

Our sincerest congratulations to Katarzyna for an exceptional Ph.D. dissertation defense!

Katarzyna pushed the boundaries of our knowledge of muscular exophergenesis in C. elegans, and her efforts resulted in three articles in this field, one of which made it to the cover of EMBO Reports.

Furthermore, Katarzyna has co-authored two other publications on the ubiquitin-proteasome system and has shared her findings at various international conferences. Thank you Katarzyna for your valuable contribution to our projects and a common scientific journey!

March 2023 | Visit of high school students

Visit of high school students

Our lab hosted a group of high school students, to whom we introduced the fascinating C. elegans nematode, a tiny worm used as a model organism in many scientific studies, including our research on cellular adaptation to cold under the NCN GRIEG grant. We hope our presentation sparked their curiosity and inspired them to pursue a career in science.

March 2023 | Ph.D. defense of Aniruddha

Ph.D. defense of Aniruddha

Huge congratulations to Aniruddha on an outstanding Ph.D. dissertation defense!

Aniruddha's research on the synergistic cooperation between the ubiquitin ligases CHN-1 and UFD-2 resulted in a first-author article that landed on the cover of the EMBO Journal and formed the basis of his Ph.D. thesis. During doctoral studies, Aniruddha also presented his results at several international conferences and participated in other projects in the lab. Aniruddha will continue his research on the ubiquitin-proteasome system as a post-doc at the University of Texas at Austin in Prof. Andreas Matouschek's group.

February 2023 | Celebrating
#WomenInSTEM Day

Natalia's interview panel

Thanks to the EU-LIFE alliance of leading European life sciences research centers, our Ph.D. student Natalia gave an interview about the challenges of ensuring equal opportunities in science on the occasion of the International Day of Women and Girls in Science #WomenInSTEM.
In Pokrzywa lab, we fully support women and promote equality in all aspects.

February 2023 | Our presence at the Rare Disease Conference

Aging iron impact

On the occasion of the upcoming Rare Diseases Day, our Ph.D. student Natalia had the pleasure of presenting at the 6th National Scientific Conference on Rare Diseases in the 21st Century our research using the nematode C. elegans, in which we demonstrated that an ultra-rare mutation in the FEM1C gene causes neurodevelopmental disorders.
We hope that our study will contribute to the development of personalized therapies for patients with mutations in FEM1C.

February 2023 | Konrad admitted to the Ph.D. program

Photo of Konrad

Congratulations to our former master's student Konrad on his admission to the Ph.D. program. Konrad will conduct his doctoral research under the supervision of an ERC grantee, Dr. Adam Kłosin, at the Nencki Institute of Experimental Biology. 

Konrad, we are very proud of you and wish you many successes and impactful discoveries!

February 2023 | New article
published

Aging iron impact

Together with the group of Dr. Katarzyna Mleczko-Sanecka from our Institute, we have published an article showing that defects in red pulp macrophages - cells responsible for maintaining blood homeostasis - occur in early aging and disrupt iron turnover, contributing to anemia in elderly age.

We are very proud of this intra-institute collaboration and look forward to more thrilling joint research!

January 2023 | New preprint
published

Lysine desert definitions

We have published a preprint describing our research on lysine-less proteins, so-called lysine deserts. We explored their prevalence, evolutionary conservation, and possible functional roles in prokaryotes and eukaryotes. In addition, using the example of lysine-free variants of VHL and SOCS1 proteins, we showed that they can undergo non-lysine ubiquitination and proteasome-dependent turnover which is independent of their ubiquitination.

January 2023 | New article
published

protocol graphical abstract

We have just published a detailed protocol for quantifying muscle exophers in C. elegans. We hope that our resource will be useful to the scientific community in performing cutting-edge studies of these exciting extracellular vesicles.

December 2022 | New preprint published

exopher graphical abstract

Together with the group of Dr. Michał Turek from the Institute of Biochemistry and Biophysics, we released a preprint where we show how sex-specific pheromones and olfactory neurons regulate muscle extracellular vesicles (exophers) in C. elegans.
Our findings may imply the existence of an analogous mechanism regulating cardiomyocyte exophers, which contributes to the risk of cardiovascular disease in humans.

December 2022 | Our presence at the PhasAGE conference

CHIP protein in nucleolus

We congratulate our senior researcher Małgorzata on giving a talk about nucleolar proteostasis control at the Biomolecular condensates in biology and disease conference organized by the PhasAGE consortium in Brussels. We hope our data shed some light on the relation between CHIP ubiquitin ligase and nucleolus in cellular stress and phase separation.

November 2022 | A Fulbrighter from our lab

Fulbright cover

We would like to congratulate our Ph.D. student Natalia on receiving the prestigious Fulbright Junior Research Award for the research stay at the Dana-Farber Cancer Institute in Boston.

November 2022 | New preprint
published

Putative degron sites in filaggrin protein

Together with the group of Dr. Danuta Owsiak-Gutowska from the University of Gdańsk, we released a preprint where we shed some light on the degradation routes of filaggrin, an important protein in the pathogenesis of atopic dermatitis.

We show the involvement of the proteasome in filaggrin turnover and we describe filaggrin's potential degron sites and their effect on its mutated variants associated with the disease.

November 2022 | New article
published

Aldicarb sensitivity assay

We are pleased that our collaboration with Prof. Rafał Płoski from the Medical University of Warsaw and Prof. Maria Mazurkiewicz-Bełdzińska from the Medical University of Gdańsk has resulted in a joint publication where we link an uncharacterized neurological disease with an ultra-rare mutation in FEM1C ubiquitin ligase.
In our article, we present the first evidence from an animal model, Caenorhabditis elegans nematode, suggesting that a mutation in the conserved FEM1C Asp126 position causes a neurodevelopmental disorder in humans.

October 2022 | Our presence
at the Rare Disease Congres

Wojciech presenting at the Rare Disease Congres

We are very grateful for the invitation to the Rare Disease Congres in Cracow from the Jesteśmy Pod Ścianą Foundation, an NGO supporting patients with rare diseases. Our PI, Wojciech, and Ph.D. student, Natalia, presented our research on an uncharacterized neurodevelopmental disorder caused by an ultra-rare mutation in the FEM1C ubiquitin ligase and chorea-acanthocytosis. We hope that our research will contribute to a better understanding of the molecular mechanisms underlying these diseases.

October 2022 | Our presence
at the FNP conference

Wojciech at the FNP conference

Our PI, Wojciech, presented the poster about our study that hit the cover of the prestigious EMBO Journal at the 4th Interdisciplinary FNP Conference, organized by our funding agency - the Foundation for Polish Science. We are very grateful for the obtained funds which enabled our research and hope for more exciting discoveries.

September 2022 | Lecture at the 26th Science Festival in Warsaw

Science Festival in Warsaw

Like last year, we were delighted to participate in the Science Festival in Warsaw, a well-recognized science popularization event in Poland. We unveiled some of the secrets of Caenorhabditis elegans and discussed its role in aging research. Students also had an opportunity to test their knowledge as we prepared a quiz with prizes.

We hope that we passed some of our fascination with this tiny creature to our listeners, and our lecture provided insight into the complex mechanisms of aging and the role of Caenorhabditis elegans in studying them.

September 2022 | M.Sc. thesis defense

Konrad after his defence

Congratulations to our M.Sc. student Konrad for graduating with a Master’s degree in Biotechnology at the University of Warsaw!
Konrad wrote his thesis about the role of human CHIP ubiquitin ligase in nucleolar protein recovery after heat stress and co-authored our recent preprint. We are very proud of you and wish you many more successes!

September 2022 | New grant
awarded

Logo of the Ministry of Education and Science

We are delighted to announce that we were awarded the Social Responsibility of Science (Społeczna Odpowiedzialność Nauki) grant from the Ministry of Education and Science for developing an educational computer game about protein degradation processes in the cell.
As we believe that science should break out of its ivory tower and be widely accessible, the aim of the proposed computer game would be to explain how cells maintain protein homeostasis and why its disruption may lead to disease. Stay tuned!

September 2022 | Our presence
at the C. elegans conference

Worm Meeting Basel

Katarzyna, our Ph.D. student, gave a talk on our soon-to-be-released study on muscular exopheresis in Caenorhabditis elegans at the Basel Worm Meeting.

We are delighted that it gained a lot of attention of the worm community and cannot wait to publish it.

September 2022 | Best M.Sc. thesis in bioinformatics

Natalia's diploma

We would like to congratulate our Ph.D. student Natalia on receiving the prestigious 1st place in the contest organized by the Polish Bioinformatics Society for the best master’s thesis in bioinformatics defended in 2021 in Poland!

September 2022 | Our presence
at the EMBO conference

Natalia at the conference in Cavtat

Our recently released story about the interplay of CHIP - UFD-2 ubiquitin ligases and our web server degronopedia.com have been presented by our PI Wojciech and Ph.D. student Natalia at the EMBO Workshop Ubiquitin and ubiquitin-like proteins in health and disease in Cavtat, Croatia.

We received a lot of questions and positive feedback and hope to reunite with the ubiquitin community at the next meeting.

August 2022 | New article
published

Graphical abstract

More research on CHIP ubiquitin ligase, with our substantial contribution, has been published in Molecular Cell.
In this article, we show that CHIP exists as both a dimer, which promotes protein quality control, and a monomer, which plays a role in regulating aging. The conformation switch is controlled by CHIP auto-ubiquitination.

August 2022 | New article
published

Graphical abstract

Our work, in collaboration with the group of Prof. Rafal Ciosk from the University of Oslo, has been published in Nature Communications.

We are very glad that we could participate in the study, showing that ferritin-mediated iron detoxification promotes the survival of C. elegans and mouse neurons in the cold. 


The article can be found here

August 2022 | Our article on the cover of EMBO Journal

EMBO cover

Our article on the CHIP - UFD-2 ubiquitin ligase interplay and its role in regulating lipid metabolism has been featured on the cover of the EMBO Journal.

We imagine UFD-2 as a jazzman who promotes cooperation between CHIP and ubiquitin-coupling enzymes, doubling the activity of CHIP - a double bell saxophone releasing ubiquitin. Through this graphic, we also wanted to pay tribute to the famous Warsaw Jazz Jamboree, which we adore.


Our article can be found here.

July 2022 | Our presence at the
C. elegans conference

Poster at the European Worm Meeting

Our new follow-up study on muscular exopheresis in Caenorhabditis elegans has been presented at the European Worm Meeting in Vienna.
Katarzyna, our Ph.D. student, lifted the veil of secrecy by showing some of our exciting results, which we will soon release as a preprint.  

June 2022 | New article published              

Graphical abstract

Our article has just been published in the prestigious EMBO Journal. We are thrilled, as it will be featured on the cover of its August issue!

We focused on the CHIP ubiquitin ligase enzyme – a quality control factor responsible for the regulation and degradation of multiple protein targets, including those implicated in the pathogenesis of neurodegenerative diseases or cancer. We described a potential regulatory process enabling switching on/off activity of CHN-1, a worm analog of human CHIP, to maintain cellular homeostasis. We also identified a set of its new potential substrates, including AHCY‐1, a key enzyme involved in methylation - a process responsible e.g., for determining if particular DNA instructions would be processed or regulating proteins and lipids.

June 2022 | Degronopedia hit 1000 queries

conference photo

We are delighted that our recently released web server degronopedia.com has just passed 1000 queries!

Moreover, our PhD student Natalia gave two successful talks about it - at the EMBO Workshop Protein Termini in Bergen, Norway and virtually at the Dana-Farber Seminar Series. We are looking forward to introduce some new features soon, so stay tuned!

May 2022 | New preprint
published

DEGRONOPEDIA logo

We published a preprint where we introduce DEGRONOPEDIA - a web server allowing for degron screening.
Degrons are short linear motifs critical for selective protein degradation. Our tool provides complex degron information (i.a. nearby residues that may be ubiquitinated, post-translational modifications, occurrence within a disorder region) and allows for Machine Learning predictions of protein termini stability.

DEGRONOPEDIA can be freely accessed at degronopedia.com 

May 2022 | Our presence at two major conferences

Travel grant awardees

Our study on CHIP/UFD-2 molecular cooperation has been presented at the Ubiquitin & Friends Symposium in Vienna and GRK2243 Symposium in Würzburg by our talented Ph.D. students - Aniruddha Das and Pankaj Thapa.
We are very pleased how warmly it was received by the scientific community as expressed by the Travel Grant awarded to Aniruddha for one of the best presented posters. 

Photo: @SFB_TPDvienna, Twitter

May 2022 | New preprint
published

CHIP scheme

We released a preprint where we describe the engagement of the ubiquitin ligase CHIP by the nucleolus in response to proteotoxic stress.
These observations may be relevant in elucidating the mechanisms of nucleolar proteostasis and in the biology of cancer, whose development is influenced by nucleolar stress response pathways. Our study provides a solid basis for further exploring CHIP function in the nucleolus.

April 2022 | New preprint
published

Omega bends plot

We released a preprint describing the first case of neurodevelopmental disorder in a patient with a de novo missense variant in a degron-binding pocket of ubiquitin ligase FEM1C. We used Caenorhabditis elegans as a model of the disease; mutant worms, similarly to the patient, also developed locomotion impairments.

This study was carried out in collaboration with the group of Prof. Rafał Płoski from the Medical University of Warsaw. We are looking forward to further fruitful collaborative research on possible mechanisms of pathogenicity underlying this mutation.

February 2022 | New grant
awarded

NCN logo

We are very pleased to inform that PI of our group - Wojciech Pokrzywa has obtained the five-year SONATA-BIS grant from the National Science Centre.

In the next years we will focus on mechanisms of protein adaptation to escape premature degradation by the ubiquitin-proteasome system. We are very excited for this new avenue of research!

January 2022 | New preprint
published

Preprint figure

In our recent preprint, we show that defects in red pulp macrophages - cells responsible for maintaining blood homeostasis - occur early in aging and disrupt iron turnover, contributing to elderly anemia.

November 2021 | New grants
awarded

PRELUDIUM grant awardees

We are delighted to announce that two of our Ph.D. students received their own PRELUDIUM grants from the National Science Centre (NCN).

Natalia Szulc will study how the cullin-RING ubiquitin ligase complexes avoid premature degradation, and Pankaj Thapa will investigate the molecular mechanism of the activity switch of the ubiquitin ligase CHIP.

September 2021 | Lecture at the 25th Science Festival in Warsaw

Science Festival 2021

We were honored to participate in the 25th Science Festival in Warsaw, one of the most significant popular science events in Poland. High school students warmly received our presentation on the secrets of Caenorhabditis elegans and its important role in aging research.
We hope that this tiny creature intrigued our listeners, and our lecture provided an overview of the current state of knowledge on mechanisms of aging.

August 2021 | Our research on the cover of EMBO reports

August 2021 EMBO reports cover

Our research on muscular exopheresis in Caenorhabditis elegans is featured as a cover of the August issue of EMBO Reports!
Here we show that exophers are not only a storage compartment for cellular waste, but that muscular exopheresis represents a previously uncharacterized nutrient management program associated with nourishing the next generation of progeny. 

August 2021 | New preprint
published

Scheme of role of UFD-2/CHN-1 complex in lipid synthesis

What drives the prominent quality control ubiquitin ligase CHIP? Our new preprint on bioRxiv describes the mechanism by which CHIP is activated by another ubiquitin ligase, UFD-2, which enables the regulation of proteostasis and lipid metabolism.

Our results define the mechanism of synergistic cooperation of CHIP and UFD-2, which promotes structural gain of function in CHIP and regulates S-adenosylhomocysteinase, an enzyme crucial for cellular methylation and phosphatidylcholine synthesis.

July 2021 | New article
published

Scheme of mechanical stress protection system

During critical moments like differentiation, adhesion, and migration cells use sophisticated mechanisms that protect them from mechanical stress. Moreover, these mechanisms are also essential for maintaining tissues such as skeletal muscle, heart, and kidney as well as the immune system.
Here you can find our article, where we discuss recent advances in our understanding of mechanical stress protection.


July 2021 | Extension of financing

Research unit FOR 2743 logo

The research unit FOR 2743 integrates mechanobiology and proteostasis research to elucidate how cells and tissues cope with force-induced protein unfolding. We identified mechano-protective proteostasis factors and signalling networks that regulate responses to mechanical stress.
Thanks to the extended funding we will further decipher principles of mechanical stress protection under physiological and pathophysiological conditions.

You can find more information here and here

March 2021 | New article
published   

Scheme of UBB+1 level on cell fitness

Our article on the different cellular effects of the intriguing mutant ubiquitin protein UBB+1 was published in Frontiers in Molecular Biosciences.
You can read here about how UBB+1 may extend lifespan, while on the other hand also leads to aggregation of toxic proteins.

January 2021 | NCN GRIEG grant opened 

NCN and Norway grants logos

Project Cellular adaptation to cold, funded by the NCN GRIEG grant & the Norwegian Financial Mechanism 2014 – 2021 for 1.499.998 EUR was opened.

We cannot wait to start the studies, together with our partner from the University of Oslo Prof. Rafał Ciosk, on how Caenorhabditis elegans can resist low temperature.

December 2020 | Extension of financing

EMBO logo

We received extension of the EMBO Installation Grant for the next 2 years!

Address

Laboratory of Protein Metabolism

International Institute of Molecular and Cell Biology in Warsaw

4 Ks. Trojdena Street

02-109 Warsaw, Poland 

Pokrzywa Lab logo
IIMCB logo